Keratin K7 polyclonal, anti-human

Quantity: 25 µl

Background:
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described.

Synonyms:
K7, CK7, SCL, K2C7

Isotype: Guinea pig Polyclonal (K7.1)

Immunogen:
Synthetic peptide (C-SAGPGLLKAYSIRT) of human keratin K7 (formerly also designated cytokeratin 7), coupled to KLH

Form: Stabilized Serum

Specificity:
Basic human keratin K7 (Mr 54 000). Detects specific subtypes of adenocarcinomas: Adenocarcinoma of pancreas, gallbladder, lung, cervix; cholangio carcinoma of liver; ductal and lobular carcinoma of breast; carcinomas of ovary; transitional cell carcinoma of bladder; mesothelioma; negative with most cases of hepatocellular carcinoma. In colorectal carcinoma early stages are reported to be negative but advanced stages of tumor development are positive for keratin K7 expression.

Reactivity: human

Applications: Immunohistochemistry on frozen and paraffin (after microwave treatment) sections, Immunoblotting (western, ECL), Cytological material

Working DIlution: 1:200 for frozen sections; 1:100 for paraffin sections; 1:3000 for Immunoblotting

Positive Control:
Glandular epithelia (e.g. sweat glands, sebaceous glands); Merkel cells

Storage: 2-8C

References:
- Moll, R. et al. (1982) Cell 31: 11-24
- Ramaekers, F. et al. (1990) Am. J. Pathol. 136: 641-655