Keratin K71 (K6irs1) polyclonal, anti-human, mouse

Quantity: 25 µl

Background:
Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins. This gene encodes a protein that is expressed in the inner root sheath of hair follicles. The type II keratins are clustered in a region of chromosome 12q13.

Synonyms:
KRT71, K6IRS1, KRT6IRS, KRT6IRS1

Isotype: Guinea pig Polyclonal (GPK6irs1)

Immunogen:
Synthetic peptide of human keratin K71 (formerly also designated keratin K6irs1; C-GGE GRS RGS AND YKD T), coupled to KLH

Form: Stabilized Serum

Specificity:
Specific for human keratin K71 (57.3 kD polypeptide; calculated from sequence data).The antiserum stains specifically the Henle, Huxley and cuticle layer of the inner root sheath (irs) of the hair follicle. Although there is a considerable sequence homology of keratin K71 to keratins K5 andK 6, no cross-reactivity was observed with Western blotting and immunofluorescence microscopy.

Reactivity: human, mouse

Applications: Immunohistochemistry on frozen sections, Immunoblotting

Working DIlution: 1:100 to 1:200 for immunohistochemistry; 1:2,000 for immunoblotting (ECL method)

Storage: 2-8C

References:
- Langbein L. et al. (1999). J Biol Chem 274: 19874 – 19893
- Langbein L. (2004) Methods in Cell Biology 78: 413-456
- Langbein L, Schweizer J (2005) Intern Rev Cytology 243: 1-78