Keratin K72 (K6irs2) polyclonal, anti-human, mouse

Quantity: 25 µl

Background:
Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells. The type II keratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This gene encodes a type II keratin that is specifically expressed in the inner root sheath of hair follicles. The type II keratins are clustered in a region of chromosome 12q12-q13. Multiple transcript variants encoding different isoforms have been found for this gene.

Synonyms:
KRT72, KRT6, K6irs, K6IRS2, KRT6IRS2

Isotype: Guinea pig Polyclonal (GPK6irs2)

Immunogen:
Synthetic peptide of human keratin K72 (formerly alsodesignated K6irs2; SYKTAAADVKTK GSC), coupled to KLH

Form: Stabilized Serum

Specificity:
The antiserum stains specifically the cuticle layer of the inner root sheath (irs) of the hair follicle.Although there is a considerable sequence homology of keratin K72 polypeptide to keratins K5 and K6, no crossreactivity was observed with Western blotting and immunofluorescence microscopy.

Reactivity: human, mouse

Applications: Suitable for immunohistochemistry on frozen sections, immunoblotting

Working DIlution: 1 : 100 - 200 for immunohistochemistry; 1 : 2,000 for Western blotting (ECL)

Storage: 2-8C

References:
- Langbein L et al. (2006) J Invest Dermatol 126: 2377-2386