Keratin K8 polyclonal, anti-human, mouse

Quantity: 25 µl

Background:
This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene.

Synonyms:
K8, KO, CK8, CK-8, CYK8, K2C8, CARD2

Isotype: Guinea pig Polyclonal (K8.2)

Immunogen:
Synthetic peptide (C-MSIRVTQKSYKVST) of human keratin K8 (formerly also designated cytokeratin 8), coupled to KLH

Form: Stabilized Serum

Specificity:
Basic human keratin K8 (Mr 52 000) of all simple type and complex epithelia. Specific for keratin K8. Marker for discrimination of simple / non-stratified epithelia from those of stratified origin.

Reactivity: human, mouse

Applications: Immunohistochemistry on frozen and paraffin sections (after microwave treatment or trypsin), Frozen tissue, Immunoblotting (western, ECL), Cytological material

Working DIlution: 1:200 for frozen sections; 1:100 for paraffin sections; 1:3000 for Immunoblotting

Positive Control:
Human intestinal epithelia

Storage: 2-8C

References:
- Moll, R. et al. (1982) Cell 31: 11-24