Ki67 polyclonal, anti-human, mouse, rat

Background:
Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface. Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility. Binds DNA, with a preference for supercoiled DNA and AT-rich DNA. Does not contribute to the internal structure of mitotic chromosomes. May play a role in chromatin organization. It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).

Alternative Name:
Antigen KI-67, Antigen Ki67, MKI67, Antigen identified by monoclonal antibody Ki 67, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67, Antigen KI67, Antigen Ki67, KI67, KIA, Marker of proliferation Ki-67, MIB 1, MIB, MKI67, PPP1R105, Proliferation marker protein Ki-67, Proliferation related Ki 67 antigen, Protein phosphatase 1 regulatory subunit 105, RP11-380J17.2,

Application Dilution:
WB:1:500
IHC:1:50-1:200

Specificity: Ki67 polyclonal antibody detects endogenous levels of Ki67 protein.

Immunogen:
Peptide

MW: 359 kDa

Swiss-Prot: P46013(Human)

Purification & Purity:
Peptide affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.