KIF3A polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
Kinesin-like protein KIF3A is a protein that in humans is encoded by the KIF3A gene. KIF3A is one subunit of the heterotrimeric motor protein, kinesin-2, that was initially isolated from sea urchin egg/embryo cytosol using microtubule affinity purification. This motor consists of two kinesin-related subunits (called KIF3A and KIF3B or 3C in vertebrates) and an associated protein (KAP3), and it transports protein complexes, nucleic acids and organelles towards the "plus" ends of microtubule tracks within cells. Work done in a broad range of eukaryotic cells has revealed that heterotrimeric kinesin-2 is the primary motor protein driving the intra-flagellar transport of tubulins and other axonemal building blocks from the base of the ciliary/flagellar axoneme to their site of assembly at the distal tips. This process is required for cilium assembly/maintenance and cilium-based signalling which play key roles in various cell and developmental processes. For example, in vertebrate embryos, kinesin-2 function is required for cilia-dependent nodal flow and the development of left-right asymmetry.

Description:
Rabbit IgG polyclonal antibody for Kinesin-like protein KIF3A (KIF3A) detection. Tested with WB in Human, Mouse, Rat.

Synonyms:
KIF 3 antibody, KIF 3A antibody, KIF3 antibody, Kif3a antibody, KIF3A_HUMAN antibody, Kifl antibody, Kinesin family member 3A antibody, Kinesin family protein 3 antibody, Kinesin family protein 3A antibody, Kinesin like protein KIF 3A antibody, Kinesin like protein KIF3A antibody, Kinesin-like protein KIF3A antibody, Kns3 antibody, Microtubule plus end directed kinesin motor 3A antibody, Microtubule plus end-directed kinesin motor 3A antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
E.coli-derived human KIF3A recombinant protein (Position: D485-Q699). Human KIF3A shares 97.7% amino acid (aa) sequence identity with mouse KIF3A.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, mouse, rat

Applications: WB

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.