KLF5 polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
KLF5(Krueppel-like factor 5) is a protein that in humans is encoded by the KLF5 gene. It is also known as BASIC TRANSCRIPTION ELEMENT-BINDING PROTEIN 2 or BTEB2. This gene encodes a member of the Kruppel-like factor subfamily of zinc finger proteins. Suske et al.(2005)stated that the human KLF5 gene maps to chromosome 13q21.33, and the mouse Klf5 gene to chromosome 14E2.1. Sogawa et al.(1993)isolated a cDNA encoding BTEB2. The predicted 219-amino acid protein contains 3 consecutive zinc finger motifs near the C terminus. The zinc finger domains were 59% and 64% identical to those of Sp1(189906) and BTEB1, respectively. Both BTEB2 and Sp1 have a short basic region preceding the zinc finger motifs that may play an auxiliary role in DNA binding. The N-terminal region of BTEB2 is proline rich. Recombinant BTEB2 protein and Sp1 showed very similar DNA-binding specificity in vitro. When expressed in mammalian cells, BTEB2 activated the transcription of a reporter gene fused to a GC box-containing promoter. Sogawa et al.(1993) also identified the rat BTEB2 homolog. Northern blot analysis of rat tissues revealed that BTEB2 is expressed only in the testis and placenta.

Description:
Rabbit IgG polyclonal antibody for Krueppel-like factor 5 (KLF5) detection. Tested with WB in Human, Mouse, Rat.

Synonyms:
Basic transcription element binding protein 2 antibody, Basic transcription element-binding protein 2 antibody, BTE binding protein 2 antibody, BTE-binding protein 2 antibody, BTEB 2 antibody, BTEB2 antibody, CKLF antibody, Colon krueppel like factor antibody, Colon krueppel-like factor antibody, GC box binding protein 2 antibody, GC-box-binding protein 2 antibody, IKLF antibody, Intestinal enriched krueppel like factor antibody, Intestinal-enriched krueppel-like factor antibody, KLF 5 antibody, Klf5 antibody, KLF5_HUMAN antibody, Krueppel like factor 5 antibody, Krueppel-like factor 5 antibody, Kruppel like factor 5 intestinal antibody, Transcription factor BTEB 2 antibody, Transcription factor BTEB2 antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence in the middle region of human KLF5(109-126aa HKKYRRDSASVVDQFFTD), identical to the related rat and mouse sequences.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human.
Predicted to work with: mouse, rat.

Applications: WB

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.