KLF8 polyclonal, anti-human

Quantity: 100 µg

Background:
KLF8(Krueppel-like factor 8) is a protein that in humans is encoded by the KLF8 gene. It is also named as ZNF741, or BKLF3. Kruppel-like factor proteins, including KLF8, all contain a characteristic C-terminal DNA-binding domain containing 3 Kruppel-like zinc fingers, and they all recognize CACCC and GC boxes present in promoters and enhancers. The International Radiation Hybrid Mapping Consortium mapped the KLF8 gene to the X chromosome(WI-18614). Suske et al.(2005) stated that the human KLF8 gene maps to chromosome Xp11.21, and the mouse Klf8 gene to chromosome XF3. van Vliet et al.(2000) identified a cDNA encoding KLF8, also called ZNF741. Expression of KLF8 or only its zinc fingers showed that the fingers could bind to a CACCC box. Sequence analysis predicted that the 359-amino acid protein contains a nuclear localization signal upstream of the zinc fingers, a valine-rich hydrophobic stretch, and a pro-val-asp-leu-ser(PVDLS) motif. Yeast 2-hybrid and GST pull-down analysis demonstrated that CTBP2 interacts with KLF8 through the PVDLS motif.

Description:
Rabbit IgG polyclonal antibody for Krueppel-like factor 8 (KLF8) detection. Tested with WB in Human.

Synonyms:
Basic krueppel-like factor 3 antibody, BKLF3 antibody, DXS741 antibody, KLF8 antibody, KLF8_HUMAN antibody, Krueppel-like factor 8 antibody, Kruppel like factor 8 antibody, MGC138314 antibody, Zinc finger protein 741 antibody, ZNF741 antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the N-terminus of human KLF8(1-18aa MVDMDKLINNLEVQLNSE).

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human

Applications: WB

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.