Klotho polyclonal, anti-human

Klotho polyclonal, anti-human

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In stock
SKU
AC-ABG10332
Catalog Number: AC-ABG10332
Size(s): 50 µg, 100 µg
Host: Rabbit
Applications: WB, E
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Source:
Produced from sera of rabbits pre-immunized with highly pure recombinant Human Klotho. Anti-Human Klotho specific antibody was purified by affinity chromatography employing immobilized Human Klotho matrix

Western Blot:
To detect hKlotho by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hKlotho is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Sandwich ELISA:
To detect hKlotho by sandwich ELISA (using 100 μl/welll antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems Biotinylated Anti-Human Klotho as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hKlotho.
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