Ku80 polyclonal, anti-human

Quantity: 100 µg

Background:
XRCC5(X-ray Repair, Complementing Defective, In Chinese Hamster, 5), also known as Ku80 or Ku86, is a protein that in humans, is encoded by the XRCC5 gene. The XRCC5 gene encodes the 80-kD subunit of the Ku autoantigen, a heterodimer which contributes to genomic integrity through its ability to bind DNA double-strand breaks and facilitate repair by the nonhomologous end joining(NHEJ) pathway. The XRCC5 gene is mapped to 2q35. Human colon cancer cells heterozygous for Ku86 are haploinsufficient with an increase in polyploid cells, a reduction in cell proliferation, elevated p53 levels, and a slight hypersensitivity to ionizing radiation. Functional inactivation of the second Ku86 allele results in cells with a drastically reduced doubling time. The Ku86 locus is essential in human somatic tissue culture cells by experiments demonstration. A rare microsatellite polymorphism in XRCC5 is associated with cancer in patients of varying radiosensitivity.

Description:
Rabbit IgG polyclonal antibody for X-ray repair cross-complementing protein 5 (XRCC5) detection. Tested with WB, IHC-P, ICC in Human.

Synonyms:
86 kDa subunit of Ku antigen antibody, ATP dependent DNA helicase 2 subunit 2 antibody, ATP dependent DNA helicase II 80 kDa subunit antibody, ATP dependent DNA helicase II 86 Kd subunit antibody, ATP dependent DNA helicase II antibody, ATP-dependent DNA helicase 2 subunit 2 antibody, ATP-dependent DNA helicase II 80 kDa subunit antibody, CTC box binding factor 85 kDa antibody, CTC box-binding factor 85 kDa subunit antibody, CTC85 antibody, CTCBF antibody, DNA repair protein XRCC5 antibody, KARP 1 antibody, KARP1 antibody, Ku 80 antibody, Ku autoantigen 80kDa antibody, Ku80 antibody, Ku86 antibody, Ku86 autoantigen related protein 1 antibody, KUB 2 antibody, KUB2 antibody, Lupus Ku autoantigen protein p86 antibody, NFIV antibody, Nuclear factor IV antibody, Thyroid lupus autoantigen antibody, Thyroid-lupus autoantigen antibody, TLAA antibody, X ray repair complementing defective repair in Chinese hamster cells 5(double strand break rejoining) antibody, X-ray repair complementing defective repair in Chinese hamster cells 5(double-strand-break rejoining) antibody, X-ray repair cross-complementing protein 5 antibody, Xray repair complementing defective repair in Chinese hamster cells 5 antibody, XRCC 5 antibody, XRCC5 antibody, XRCC5_HUMAN antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the C-terminus of human Ku80(648-662aa KFSEEQRFNNFLKAL).

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human

Applications: WB, IHC-P, ICC

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.