Ku80 polyclonal, anti-human

Background:
The Ku protein is localized in the nucleus and is composed of subunits referred to as Ku-70 (p70) and Ku-86 (p86) which is also known by the synonym Ku-80 or (p80). Ku was first described as an autoantigen to which antibodies were produced in a patient with scleroderma polymyositis overlap syndrome, and was later found in the sera of patients with other rheumatic diseases. Both subunits of the Ku protein have been cloned, and a number of functions have been proposed for Ku, including cell signaling, DNA replication and transcriptional activation. Ku is involved in Pol II-directed transcription by virtue of its DNA binding activity, serving as the regulatory component of the DNA-associated protein kinase that phosphorylates Pol II and transcription factor Sp. Ku proteins also activate transcription from the U1 small nuclear RNA and the human transferrin receptor gene promoters. A Ku-related protein designated the enhancer 1 binding factor (E1BF), composed of two subunits, has been identified as a positive regulator of RNA polymerase I transcription initiation.

Alternative Name:
CTC85, CTCBF, TLAA, XRCC5, 86 kDa subunit of Ku antigen, ATP dependent DNA helicase 2 subunit 2, ATP dependent DNA helicase II 80 kDa subunit, ATP dependent DNA helicase II 86 Kd subunit, ATP dependent DNA helicase II, ATP-dependent DNA helicase 2 subunit 2, ATP-dependent DNA helicase II 80 kDa subunit, CTC box binding factor 85 kDa, CTC box-binding factor 85 kDa subunit, CTC85, CTCBF, DNA repair protein XRCC5, KARP 1, KARP1, Ku 80, Ku autoantigen 80kDa, Ku80, Ku86, Ku86 autoantigen related protein 1, KUB 2, KUB2, Lupus Ku autoantigen protein p86, NFIV, Nuclear factor IV, Thyroid lupus autoantigen, Thyroid-lupus autoantigen, TLAA, X ray repair complementing defective repair in Chinese hamster cells 5 (double strand break rejoining), X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining), X-ray repair cross-complementing protein 5, Xray repair complementing defective repair in Chinese hamster cells 5, XRCC 5, XRCC5, XRCC5,

Application Dilution:
WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200

Specificity: Ku80 polyclonal antibody detects endogenous levels of Ku80 protein.

Immunogen:
recombinant protein

MW: 83 kDa

Swiss-Prot: P13010(Human)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.