L-Lactic Acid/Lactate (LA) Colorimetric Assay Kit
€0.00
In stock
SKU
E-BC-K044-M
Catalog Number: E-BC-K044-M
Assay Type: Quantitative
Detection Range: 0.12-7.0 mmol/L, Sensitivity: 0.10 mmol/L
Datasheet
Questions? Contact us!
Assay Type: Quantitative
Detection Range: 0.12-7.0 mmol/L, Sensitivity: 0.10 mmol/L
Datasheet
Questions? Contact us!
Detection Principle:
Using NAD+ as H+ receptor, LDH catalyzes the reaction of lactic acid and NAD+ to generate pyruvic acid and NADH respectively. NBT is reduced to a kind of purple compound during the reaction. Measure the OD value at 530 nm, and the concentration of lactic acid can be calculated.
Instrument: Microplate reader
Assay Time: 30 min
Tested Sample Types: Serum,plasma,cell culture supernatant,tissue,cells
Research Area's: Glycolysis & Carbohydrates
Shelf Life: 6 Months
Storage:
2-8℃
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