L1CAM (Extracellular) (clone M023), anti-human

Background:
The neural cell adhesion molecule L1 (L1CAM/NCAM-L1/CD171) is a single pass transmembrane glycoprotein member of the immunoglobulin superfamily that contains six Ig-like domains and five fibronectin type-III domains in the extracellular region. L1CAM is mainly expressed in the brain where it has important roles in the developing nervous system. L1CAM has been implicated in neuronal migration and adhesion, axon myelination and fasciculation, and neurite outgrowth. Mutations in the L1CAM gene cause varying degrees of neurological disease including X-linked hydrocephalus, MASA syndrome, spastic paraplegia type 1, and X-linked corpus callosum agenesis. Apart from the nervous system, L1CAM is overexpressed in many cancers and supports a poor prognosis by facilitating aggressive tumor growth, metastasis, and chemoresistance.

Background References
Moos, M. et al. (1988) Nature. 334:701.
Hortsch, M. (1996) Neuron. 17:587.
Angiolini F, Cavallaro U (2017) Int J Mol Sci 18(2)pii:E254.

Immunogen: Clone (M023) was generated from a proprietary antigen related to the native human L1CAM expressed in the MeWo melanoma cell line.

Specificity: Clone M023 mouse monoclonal antibody detects a 230 kDa* protein on SDS-PAGE immunoblots of human MeWo, MDA-MB-231, A431, and MCF7 cells, as well as human brain tissue. The antibody works for western blot, immunoprecipitation, ELISA, and immunocytochemistry, as well as detects L1CAM on live cells.

Application dilution:
ELISA: 1:2000
ICC: 1:100
IP: 1:100
WB: 1:1000
End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.

Buffer/Storage:
Mouse monoclonal antibody purified with protein G chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.