Lactoferrin (clone NI 25) biotin, anti-human

Background:
The reactivity of the antiserum is restricted to lactoferrin as tested in indirect binding immunoblotting and indirect immunoperoxidase staining. To identify the presence of lactoferrin in human milk, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining and immunoblotting. As a second step an avidin or streptavidin conjugate of the user's choice has to be used. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:20 upwards; in ELISA from 1:25 upwards; in Western blotting from 1:50 upwards. These data should be interpreted as general recommendations only.

Clone: NI 25

Isotype: Mouse IgG1

Source: Highly purified lactoferrin isolated from human milk.

Reactivity: human. The anti-serum does not react with any other component of the human milk or plasma protein as tested. This anti-serum has not been tested for cross-reactivity with other species.

Specificity: Biotin-conjugated purified monoclonal Mouse antibody to Human lactoferrin.

Form: Purified monoclonal mouse IgG1 kappa conjugated with biotin, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.

Applications: ELISA, Immunocytochemistry, Immunohistochemistry (paraffin), Dot blot, Immunoblotting

Storage: Store at 4C, or in small aliquots at -20C.