Laminin alpha5 Polyclonal Antibody

Background:
Components of the extracellular matrix exert myriad effects on tissues throughout the body. In particular, the laminins, a family of heterotrimeric extracellular glycoproteins, affect tissue development and integrity in such diverse organs as the kidney, lung, skin, and nervous system. It is thought that laminins mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Laminins function as heterotrimeric complexes of alpha, beta, and gamma chains, with each chain type representing a different subfamily of proteins. The protein encoded by this gene belongs to the alpha subfamily of laminin chains and is a major component of basement membranes. Two transcript variants encoding different isoforms have been found for this gene, but the full-length nature of one of them has not been determined.

Synonyms:
LAMA5, KIAA0533, KIAA1907, Laminin subunit alpha-5, Laminin-10 subunit alpha, Laminin-11 subunit alpha, Laminin-15 subunit alpha

Immunogen: Synthesized peptide derived from the Internal region of human Laminin α-5

Swissprot: O15230

Calculated MW: 400 kDa
Observed MW: 400 kDa

Purification Method: Affinity purification

Buffer: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4

Concentration: 1 mg/mL
Dilution: WB 1:500-1:2000, IHC 1:100-1:300, ELISA 1:20000

Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Citation(s):
https://www.sciencedirect.com/science/article/pii/S0014483518305578
https://pubmed.ncbi.nlm.nih.gov/31126890/