Laminin beta1 Polyclonal Antibody

Background:
Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. This gene encodes the beta chain isoform laminin, beta 1. The beta 1 chain has 7 structurally distinct domains which it shares with other beta chain isomers. The C-terminal helical region containing domains I and II are separated by domain alpha, domains III and V contain several EGF-like repeats, and domains IV and VI have a globular conformation. Laminin, beta 1 is expressed in most tissues that produce basement membranes, and is one of the 3 chains constituting laminin 1, the first laminin isolated from Engelbreth-Holm-Swarm (EHS) tumor. A sequence in the beta 1 chain that is involved in cell attachment, chemotaxis, and binding to the laminin receptor was identified and shown to have the capacity to inhibit metastasis.

Research Area: Cancer, Cardiovascular, Signal Transduction

Synonyms:
CLM, Cutis laxa with marfanoid phenotype, LAM B1, LAMB 1, LAMB1, LAMB1, Laminin B1, Laminin B1 chain, Laminin beta 1 chain, Laminin beta 1 chain precursor, Laminin beta1, Laminin subunit beta 1, Laminin subunit beta-1, Laminin-1 subunit beta, Laminin-10 subunit beta, Laminin-12 subunit beta, Laminin-2 subunit beta, Laminin-6 subunit beta, Laminin-8 subunit beta, LIS5, MGC142015

Immunogen: Recombinant protein corresponding to Mouse Laminin β1

Swissprot: P07942,P02469

Calculated MW: 198 kDa
Observed MW: 220 kDa

Purification Method: Affinity purification

Buffer: PBS with 0.02% sodium azide, 1% BSA and 50% glycerol, pH7.4

Concentration: 1.2 mg/mL
Dilution: WB 1:500-1:2000

Storage:
Store at -20°C. Avoid freeze / thaw cycles.