Laminin gamma 1 (LAMC1) (clone SPM193), anti-human, mouse
€0.00
In stock
SKU
ARP-24-3915-RTM1X
Catalog Number: 24-3915-RTM1X
Size: 100 µl, 0.5 ml, 1 ml
Isotype: Rat IgG2a, kappa
Size: 100 µl, 0.5 ml, 1 ml
Isotype: Rat IgG2a, kappa
Decription:
Laminins are large hetero-trimeric, non-collagenous glycoproteins composed of α, β, and γ chains. This monoclonal antibody reacts with laminin B2/1 chain of ~210kDa and does not cross-react with other basement membrane components or fibronectin. Its specificity was established by immunoprecipitation and immunofluorescence of human skeletal muscle and kidney with laminin chain-specific monoclonal antibodys. Epithelial sheets in vivo are separated from the mesenchymal elements of the stroma by a thin layer of a specialized type of extracellular matrix termed the basement membrane (BM). This structure consists of individual components, some of which are ubiquitous in BMs and some are not. The ubiquitous ones comprise laminin (LN), entactin/nidogen (EN), collagen type IV (CIV), and large heparan sulfate proteoglycan (HSPG), which interact specifically with each other to form a continuous and regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms. This monoclonal antibody stains uniformly all human and murine basement membranes.
Synonyms: LAMB2, LAMC1, Laminin B2 chain, Laminin gamma 1, Laminin gamma 1 chain
Clone: SPM193
Isotype: Rat IgG2a, kappa
Immunogen: Murine EHS laminin preparation
Reactivity: human and mouse. Others-not known.
Form: Purified, in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Applications: Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 min at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Reactivity: Normal colon or carcinomas
Concentration: 0.2mg/ml
Storage: Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.
Laminins are large hetero-trimeric, non-collagenous glycoproteins composed of α, β, and γ chains. This monoclonal antibody reacts with laminin B2/1 chain of ~210kDa and does not cross-react with other basement membrane components or fibronectin. Its specificity was established by immunoprecipitation and immunofluorescence of human skeletal muscle and kidney with laminin chain-specific monoclonal antibodys. Epithelial sheets in vivo are separated from the mesenchymal elements of the stroma by a thin layer of a specialized type of extracellular matrix termed the basement membrane (BM). This structure consists of individual components, some of which are ubiquitous in BMs and some are not. The ubiquitous ones comprise laminin (LN), entactin/nidogen (EN), collagen type IV (CIV), and large heparan sulfate proteoglycan (HSPG), which interact specifically with each other to form a continuous and regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms. This monoclonal antibody stains uniformly all human and murine basement membranes.
Synonyms: LAMB2, LAMC1, Laminin B2 chain, Laminin gamma 1, Laminin gamma 1 chain
Clone: SPM193
Isotype: Rat IgG2a, kappa
Immunogen: Murine EHS laminin preparation
Reactivity: human and mouse. Others-not known.
Form: Purified, in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Applications: Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 min at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Reactivity: Normal colon or carcinomas
Concentration: 0.2mg/ml
Storage: Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.
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