LDL-Receptor (Low Density Lipoprotein Receptor) polyclonal biotin, anti-human

Quantity: 50 µl

Background:
The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants.

Synonyms:
FH, FHC, LDLCQ2

Isotype: Rabbit Polyclonal

Immunogen:
Recombinant human LDL-Receptor domain I, II, III Region

Form: Purified IgY

Specificity:
The antibody is directed against the extracellular domains I, II, III within the ligand binding region of the human LDL-Receptor.

Reactivity: human (tested so far)

Applications: FACS, Immunoblotting (western), ELISA, Receptor binding studies, Immunofluorescence

Working DIlution: 1:50 to 1:100 for Immunofluorescence; 1:50 for Immunoblotting; 1:10 for FACS

Storage: 2-8C for immediate use, or at -20C (aliquot)