LDL Receptor polyclonal, anti-human

Quantity: 50 µl

Background:
The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants.

Synonyms:
FH, FHC, LDLCQ2

Isotype: Rabbit Polyclonal

Immunogen:
Specific synthetic peptide (sequence not conserved in VLDL receptor and LRP) of the LDL receptor extracellular domain (epitope between residues # 184-195, the linker region between repeats 4 and 5)

Form: Affinity purified IgG (Immunoaffinity chromatography), in PBS with 0.5% BSA and 0.1% Sodium Azide

Specificity:
The antibody to LDL-R reacts specifically with human LDL-R and is suitable for use in flow cytometry and immunoblotting. First data show that the antibody does not inhibit binding of LDL. In Immunoblotting, the antibody recognizes the 160 kDa band of LDL-R and a 120 kDa band of LDL-R precursor from fibroblasts, hepatocytes, and monocytic cells cultured in the presence of lipoprotein deficient serum.

Reactivity: human

Applications: Immunoblotting, Flow cytometry, Immunohistochemistry, Receptor binding studies

Working DIlution: 1:10 for Flow Cytometry; 1:100 - 1:200 for immunoblotting (ECL method), 1:20 for Immunohistochemistry (membrane staining)

Storage: 2-8C for immediate use, or at -20C (aliquot)

References:
- Eden ER. et al. (2002) J Clin Invest 110: 1695-1702