Leptin (clone HLEP 55G), anti-human

Decription:
Leptin is involved in the signaling pathway that regulates the size of the body fat depot. An increase in the level of leptin may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. Defects in the leptin gene may be a cause of autosomal recessive obesity.

Synonyms: OB Gene Product, Obese Factor

Clone: HLEP 55G

Isotype: Mouse IgG2b kappa

Immunogen: Recombinant human leptin.

Reactivity: human

Specificity: Recognizes human leptin. Detects a band of ~25kDa by Western blot.

Form: Purified, in 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.

Applications:
ELISA: (direct or indirect: 1:5'000-1:10'000)
Western blot: (1:5'000-1:10'000 using ECL. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature).

Working Dilution: Optimal conditions must be determined individually for each application.

Concentration: 1mg/ml

Storage: 2-8C immediate use, or -20C (aliquote)