Leptin polyclonal, anti-mouse
€0.00
In stock
SKU
AC-ABG10340
Catalog Number: AC-ABG10340
Size(s): 50 µg, 100 µg
Host: Rabbit
Applications: WB, IHC, E
Datasheet
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Size(s): 50 µg, 100 µg
Host: Rabbit
Applications: WB, IHC, E
Datasheet
Request Information
Source:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mLeptin. Anti-Murine Leptin specific antibody was purified by affinity chromatography employing immobilized mLeptin matrix.
Western Blot:
To detect mLeptin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mLeptin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich ELISA:
To detect mLeptin by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems Biotinylated Anti-Murine Leptin (61-085BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mLeptin.
Immunohistochemistry:
This antibody stained 4% PFA, paraffin-embedded sections of Trichuris muris infected murine cecum and large intestine tissue. The primary antibody was incubated at 0.20 μg/ml overnight at 4°C and the secondary antibody was a cyanine-3 conjugated Donkey anti-Rabbit (Jackson ImmunoResearch). Heat induced antigen retrieval with a 100mM citric acid was used. Information and photo are courtesy of David Artis, University of Pennsylvania. Optimal concentrations and conditions may vary.
Formulation: A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mLeptin. Anti-Murine Leptin specific antibody was purified by affinity chromatography employing immobilized mLeptin matrix.
Western Blot:
To detect mLeptin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mLeptin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich ELISA:
To detect mLeptin by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems Biotinylated Anti-Murine Leptin (61-085BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mLeptin.
Immunohistochemistry:
This antibody stained 4% PFA, paraffin-embedded sections of Trichuris muris infected murine cecum and large intestine tissue. The primary antibody was incubated at 0.20 μg/ml overnight at 4°C and the secondary antibody was a cyanine-3 conjugated Donkey anti-Rabbit (Jackson ImmunoResearch). Heat induced antigen retrieval with a 100mM citric acid was used. Information and photo are courtesy of David Artis, University of Pennsylvania. Optimal concentrations and conditions may vary.
Formulation: A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
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