Leukotriene A4 (LTA4) Detection Kit

Use: Used to detect the concentration of leukotriene A4 (LTA4) in samples such as serum, plasma, and cell culture supernatant.Specificity: The kit uses enzyme-linked immunosorbent assay. Biotin-labeled leukotriene A4 (LTA4) and purified anti-leukotriene A4 (LTA4) antibodies are coated on microplates. In the competitive inhibition reaction, a certain amount of solid-phase antibodies and biotin-labeled leukotriene A4 (LTA4) and non-labeled antigens (calibrators or specimens) inhibit the competitive reaction. The binding amount of the antibody and biotin-labeled leukotriene A4 (LTA4) is inhibited by the amount of non-labeled antigen. The more non-labeled antigen, the less binding of the antibody and biotin-labeled leukotriene A4 (LTA4), and vice versa. After the reaction is balanced, a solid-phase antibody-biotinylated leukotriene A4 (LTA4) is formed, and then enzyme-labeled avidin is added to form a solid-phase antibody-biotinylated leukotriene A4 (LTA4)-enzyme-avidin complex. After adding substrate for color development, the absorbance (OD value) is measured at a wavelength of 450nm using an enzyme reader. As the concentration of leukotriene A4 (LTA4) increases, the OD value gradually decreases and shows a good linear relationship. This kit has the characteristics of high sensitivity, strong specificity, good repeatability, simple operation and rapidity, and has reliable detection performance for the reduction or increase of leukotriene A4 (LTA4) in samples.

Storage Conditions
Store at 2-8°C.

Use: The kit is for research use only.