Lewis a (clone 7LE), anti-human, mouse

Source:
A BALB/c mouse was immunized with ovarian cyst mucin.
Fusion partner: SP-2/0.

Specifications:
7LE reacts with Lewis a blood group antigen, a carbohydrate determinant present on both glycolipids and glycoproteins, expressed a.o. in colonic and kidney epithelial cells. Lewis a may be useful for detection of gastrointestinal, pancreatic and colorectal carcinomas.

Species reactivity:
Positive: human, mouse.

Applications:
Demonstration of Lewis a blood group in IHC, ELISA, flow cytometry, immunofluorescence and Western blot.
ELISA: +
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: itrate
Western blot: +

Positive control: Human colon.

References:
- Rouger Ph. et al. (Eds., Arnette): Proceedings of the First International Workshop on monoclonal antibodies against human red blood cells and related antigens (Paris 1987) in: Rev Fr Transfus Immunohematol.30(5):353-717 (1987).
- Creuzot-Garcher et al. Invest Oph(thalmol Vis Sci.40(8):1631-6 (1999).
- Torrado et al. Cancer Epidemiol Biomarkers Prev.1(3): 199-205 (1992).
- Torrado et al. Gastroenterology, 102 (2): 424-430 (1992)