Lewis b (clone 2-25 LE), anti-human, guinea pig

Source:
A BALB/c mouse was immunized with ALea-b+ ovarian cyst mucin.
Fusion partner: SP-2/0.

Specifications:
2-25LE reacts with Lewis b antigen which is highly expressed in stomach, colon, small intestine, lung and kidney and to a lesser extent in salivary gland, bladder, uterus and liver.

Species reactivity:
Positive: human, guinea pig.

Applications:
Demonstration of Lewis b blood group in IHC, ELISA, flow cytometry, immunofluorescence and Western blot.
ELISA: +
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: citrate
Western blot: +

Positive control: Stomach, colon, small intestine, lung and kidney, colorectal adenocarcinoma.

References:
- Bara et al. Cancer Res.46: 3983-3898 (1986).
- Rouger Ph. et al. (Eds., Arnette): Proceedings of the First International Workshop on monoclonal antibodies against human red blood cells and related antigens (Paris 1987) in: Rev Fr Transfus Immunohematol.30(5): 353-717 (1987).
- Bara et al. Biochem. J.254: 185-193 (1988).
- Creuzot-Garcher et al. Invest Oph(thalmol Vis Sci.40(8):1631-6 (1999).
- Good et al. Vox Sanguinis62(3): 180-189 (1992).
- Torrado et al. Cancer Epidemiol Biomarkers Prev.1(3): 199-205 (1992).
- Torrado et al. Gastroenterology, 102 (2): 424-430 (1992).