LFNG Polyclonal Antibody

Background:
This gene is a member of the fringe gene family which also includes radical and manic fringe genes. They all encode evolutionarily conserved glycosyltransferases that act in the Notch signaling pathway to define boundaries during embryonic development. While their genomic structure is distinct from other glycosyltransferases, fringe proteins have a fucose-specific beta-1, 3-N-acetylglucosaminyltransferase activity that leads to elongation of O-linked fucose residues on Notch, which alters Notch signaling. This gene product is predicted to be a single-pass type II Golgi membrane protein but it may also be secreted and proteolytically processed like the related proteins in mouse and Drosophila (PMID: 9187150). Mutations in this gene have been associated with autosomal recessive spondylocostal dysostosis 3. Multiple transcript variants encoding different isoforms have been found for this gene.

Research Area: Developmental Biology, Neuroscience, Stem Cells

Synonyms:
3-N-acetylglucosaminyltransferase lunatic fringe, Beta-1, Beta-13-N-acetylglucosaminyltransferase lunatic fringe, lfng, LFNG, O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase, SCDO3

Immunogen: Fusion protein of human LFNG

Swissprot: Q8NES3
Gene Accession: BC014851

Calculated MW: 42 kDa
Observed MW: Refer to figures

Purification Method: Antigen affinity purification

Buffer: PBS with 0.05% NaN3 and 40% Glycerol, pH7.4

Concentration: 1.08 mg/mL
Dilution: WB 1:500-1:2000, IHC 1:50-1:200, ELISA 1:5000-1:10000

Storage:
Store at -20°C. Avoid freeze / thaw cycles.