LIF mouse active recombinant protein
€295.00
In stock
SKU
600359
Protein Family: Cytokines
Pathway and Disease: Immune System, Signal Transduction, Signaling Molecules and Interaction
Alternate Names: Leukocyte Inhibitory Factor, LIFDescription:
Mouse Leukemia Inhibitory Factor (LIF) is member of the IL-6 family and is made by a variety of tissues. LIF signals through receptors gp190 (LIFR) and gp130 to activate STAT3 which ultimately promotes mouse embryonic stem cell self-renewal and pluripotentcy of long term cultures. This activity is similar to that attributed to FGF-basic (FGF-2) for human embryonic stem cells. Mouse LIF is produced in E.coli. Recombinant mouse LIF is a non-glycosylated protein, containing 181 amino acids, with a molecular weight of 20 kDa.
Source: E. coli. Endotoxin level as measured by LAL is <0.05ng/ug or <0.5EU/ug.
Applications: E, WB
Application Notes:
The activity is determined by its ability to inhibit proliferation of M1 cells and is typically less than 0.03 ng/mL. Specific activity: 3.3 x 10e7 units/mg
Accession No.: P09056
MW: 20000 Da
Sequence:
MSPLPITPVN ATCAIRHPCH GNLMNQIKNQ LAQLNGSANA LFISYYTAQG EPFPNNVEKL CAPNMTDFPS FHGNGTEKTK LVELYRMVAY LSASLTNITR DQKVLNPTAV SLQVKLNATI DVMRGLLSNV LCRLCNKYRV GHVDVPPVPD HSDKEAFQRK KLGCQLLGTY KQVISVVVQA F
Purity:
Purity > 95% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Format:
Each vial contains 20 ug of lyophilized protein. Reconstitute with 0.2 ml sterile deionized water for a final concentration of 0.1 mg/ml.
Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
Pathway and Disease: Immune System, Signal Transduction, Signaling Molecules and Interaction
Alternate Names: Leukocyte Inhibitory Factor, LIFDescription:
Mouse Leukemia Inhibitory Factor (LIF) is member of the IL-6 family and is made by a variety of tissues. LIF signals through receptors gp190 (LIFR) and gp130 to activate STAT3 which ultimately promotes mouse embryonic stem cell self-renewal and pluripotentcy of long term cultures. This activity is similar to that attributed to FGF-basic (FGF-2) for human embryonic stem cells. Mouse LIF is produced in E.coli. Recombinant mouse LIF is a non-glycosylated protein, containing 181 amino acids, with a molecular weight of 20 kDa.
Source: E. coli. Endotoxin level as measured by LAL is <0.05ng/ug or <0.5EU/ug.
Applications: E, WB
Application Notes:
The activity is determined by its ability to inhibit proliferation of M1 cells and is typically less than 0.03 ng/mL. Specific activity: 3.3 x 10e7 units/mg
Accession No.: P09056
MW: 20000 Da
Sequence:
MSPLPITPVN ATCAIRHPCH GNLMNQIKNQ LAQLNGSANA LFISYYTAQG EPFPNNVEKL CAPNMTDFPS FHGNGTEKTK LVELYRMVAY LSASLTNITR DQKVLNPTAV SLQVKLNATI DVMRGLLSNV LCRLCNKYRV GHVDVPPVPD HSDKEAFQRK KLGCQLLGTY KQVISVVVQA F
Purity:
Purity > 95% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Format:
Each vial contains 20 ug of lyophilized protein. Reconstitute with 0.2 ml sterile deionized water for a final concentration of 0.1 mg/ml.
Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
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