LIM Kinase 2B (LIMK2B) Antibody (N-term) Blocking peptide
€363.00
In stock
SKU
AC-BP7814a
Background:
There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The LIMK2 protein is phosphorylated and activated by ROCK, a downstream effector of Rho, and LIM kinase 2, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. Two alternative splice variants of LIMK2 that utilize alternative promoters have been identified.
Other Names:
LIM domain kinase 2, LIMK-2, LIMK2
Target/Specificity:
The synthetic peptide sequence used to generate the antibody AP7814a was selected from the N-term region of human LIMK2B . A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
Gene Name: LIMK2
Gene ID: 3985
Primary Accession: P53671
Other Accession: Q99464
Format: Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed.
There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The LIMK2 protein is phosphorylated and activated by ROCK, a downstream effector of Rho, and LIM kinase 2, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. Two alternative splice variants of LIMK2 that utilize alternative promoters have been identified.
Other Names:
LIM domain kinase 2, LIMK-2, LIMK2
Target/Specificity:
The synthetic peptide sequence used to generate the antibody AP7814a was selected from the N-term region of human LIMK2B . A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
Gene Name: LIMK2
Gene ID: 3985
Primary Accession: P53671
Other Accession: Q99464
Format: Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed.
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