LIMK1 (activated) Lysate
€175.00
In stock
SKU
ECM-LL8051
Background:
LIM kinases (LIMK1 and LIMK2) are serine/threonine kinases that have two zinc finger motifs, known as LIM motifs, in their amino-terminal regulatory domains. LIM kinases are involved in actin cytoskeletal regulation downstream of Rho-family GTPases, PAKs, and ROCK. PAK1 and ROCK phosphorylate LIMK1 or LIMK2 at the conserved Thr-508 or Thr-505 residues in the activation loop, increasing LIMK activity. In addition, VEGF-induced stress fiber formation has been linked to p38-mediated activation of LIMK through MK-2 phosphorylation of Ser-323. Activated LIM kinases inhibit the actin depolymerization activity of cofilin by phosphorylation at the amino-terminal Ser-3 residue of cofilin. An activated His-tagged mouse LIMK1 contains Thr-508 phosphorylation. The phosphorylated kinase is detected by anti-LIMK1 (N-terminus) Cat.# LP1831 and anti-LIMK1 (Thr-508) Cat.# LP1891. This activated kinase can be dephosphorylated at Thr-508 by treating with l-phosphatase for 30 min. at 37°C.
Buffer/Storage:
LIMK (activated) Lysate is supplied at a concentration of 2 ng/µl in electrophoresis sample buffer (62.5 mM Tris pH 6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% β-mercaptoethanol). Store at –20°C. Do not boil or dilute. Stable for 1 year.
LIM kinases (LIMK1 and LIMK2) are serine/threonine kinases that have two zinc finger motifs, known as LIM motifs, in their amino-terminal regulatory domains. LIM kinases are involved in actin cytoskeletal regulation downstream of Rho-family GTPases, PAKs, and ROCK. PAK1 and ROCK phosphorylate LIMK1 or LIMK2 at the conserved Thr-508 or Thr-505 residues in the activation loop, increasing LIMK activity. In addition, VEGF-induced stress fiber formation has been linked to p38-mediated activation of LIMK through MK-2 phosphorylation of Ser-323. Activated LIM kinases inhibit the actin depolymerization activity of cofilin by phosphorylation at the amino-terminal Ser-3 residue of cofilin. An activated His-tagged mouse LIMK1 contains Thr-508 phosphorylation. The phosphorylated kinase is detected by anti-LIMK1 (N-terminus) Cat.# LP1831 and anti-LIMK1 (Thr-508) Cat.# LP1891. This activated kinase can be dephosphorylated at Thr-508 by treating with l-phosphatase for 30 min. at 37°C.
Buffer/Storage:
LIMK (activated) Lysate is supplied at a concentration of 2 ng/µl in electrophoresis sample buffer (62.5 mM Tris pH 6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% β-mercaptoethanol). Store at –20°C. Do not boil or dilute. Stable for 1 year.
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