Lipoprotein lipase polyclonal, anti-bovine, human, mouse, rat

Quantity: 100 µg

Background:
Lipoprotein lipase(LPL) is the central enzyme in plasma triglyceride hydrolysis and is secreted by macrophages in the subendothelial space. Evidence has been provided that LPL produced by macrophages in the vessel wall exerts proatherogenic effects. Lipoprotein lipase has been difficult to purify, and its protein sequence remained undetermined until it could be deduced from the nucleotide sequence of its cDNA. The gene encodes a protein of 475 amino acids that becomes a mature protein of 448 residues after cleavage of a signal peptide. Analysis of the sequence indicated that human lipoprotein lipase, hepatic lipase, and pancreatic lipase are members of a gene family. The atherogenic effects of LPL have been mainly attributed to its ability to favor lipid accumulation within macrophages present in the atherosclerotic lesion.

Description:
Rabbit IgG polyclonal antibody for Lipoprotein lipase (LPL) detection. Tested with WB in Human, Mouse, Rat, Bovine.

Synonyms:
EC 3.1.1 antibody, EC 3.1.1.34 antibody, HDLCQ11 antibody, LIPD antibody, LIPL_HUMAN antibody, Lipoprotein lipase antibody, LPL antibody, LPL protein antibody, MGC137861 antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the C-terminus of human Lipoprotein lipase(448-170aa EKVSHLQKGKAPAVFVKCHDKSL), different from the related mouse and rat sequences by two amino acids.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: bovine, human.
Predicted to work with: mouse, rat.

Applications: WB

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.