Lipoxin A4 (LXA4) Detection Kit

Use: Used to detect the concentration of lipoxin A4 (LXA4) in samples such as serum, plasma, and cell culture supernatant.Specificity: The kit uses enzyme-linked immunosorbent assay. Biotin-labeled lipoxin A4 (LXA4) and purified anti-lipoxin A4 (LXA4) antibodies are coated on microplates. In the competitive inhibition reaction, a certain amount of solid-phase antibody inhibits the competitive reaction with biotin-labeled lipoxin A4 (LXA4) and non-labeled antigen (calibrator or specimen). The binding amount of antibody and biotin-labeled lipoxin A4 (LXA4) is inhibited by the amount of non-labeled antigen. The more non-labeled antigen, the less binding of antibody and biotin-labeled lipoxin A4 (LXA4), and vice versa. After the reaction is balanced, solid-phase antibody-biotinylated lipoxin A4 (LXA4) is formed, and then enzyme-labeled avidin is added to form a solid-phase antibody-biotinylated lipoxin A4 (LXA4)-enzyme label-avidin complex. After adding substrate for color development, the absorbance (OD value) is measured at a wavelength of 450nm using an enzyme reader. As the concentration of lipoxin A4 (LXA4) increases, the OD value gradually decreases and shows a good linear relationship. This kit has the characteristics of high sensitivity, strong specificity, good repeatability, simple operation and rapidity, and has reliable detection performance for the reduction or increase of lipoxin A4 (LXA4) in the sample.

Storage Conditions
Store at 2-8°C.

Use: The kit is for research use only.