Luciferase Monoclonal Antibody

Background:
Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.

Research Area: Tags and Cell Markers

Synonyms:
ec 1.13.12.7, Firefly, Luciferase, Luciferin 4 monooxygenase

Immunogen: Synthetic Peptide of Luciferase

Swissprot: P08659
Observed MW: 60 kDa

Purification Method: Protein A purification

Buffer: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4

Concentration: 1 mg/mL
Dilution: WB 1:1000-2000

Storage:
Store at -20°C. Avoid freeze / thaw cycles.