Macrophage marker (clone D11), anti-human

Source:
A BALB/c mouse was immunized with a membrane preparation from human hepatocytes.
Fusion partner: SP2/0.

Specifications:
D11 reacts specifically with human monocytes and macrophages, in all sorts of tissues. The 125/135 kDa antigen is present on the cell membrane as well as within cytoplasmic structures including lysosomes, and is different from CD68. Among tumors, histiocytomas and histiocytic lymphomas are positive. In ALL, positive reaction with D11 indicates B-lineage derivation. AML are negative.

Species reactivity:
Positive: human. Negative: mouse, pig, rat.

Applications:
D11 can be used in immunohistochemistry, immunofluorescence tests and immunoblotting.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: Citrate
Western blot: +

Positive control: Liver or histiocytoma.

References:
- Rudinskaya T.D. et al. Immunol Lett. 33(1): 1-7 (1992).
- Frenkel M.A. et al. Gematol Transfuziol. 40(4): 13-16 (1995).
- Tupitsyn N.N et al., Int J Cancer. 68(2): 160-163 (1996).
- Petrovichev N.N et al., Acta Cytol. 41(2): 357-363 (1997)