Mad2L1 polyclonal, anti-human

Background:
Cell cycle progression is subject to arrest at the mitotic spindle assembly checkpoint in response to incorrect spindle fiber assembly. MAD2 (for mitotic arrest-deficient) is a component of the mitotic spindle checkpoint. Cells with mutated MAD2 do not undergo mitotic arrest in response to incorrect spindle fiber assembly, which results in missegregation and eventual cell death. A breast carcinoma cell line with reduced MAD2 expression, T47D, was shown to complete mitosis in the presence of nocodazole, an inhibitor of mitotic spindle assembly. MAD2 is localized to unattached kinetochores during prometaphase and disassociates upon spindle fiber attachment, indicating that MAD2 regulates kinetochore binding to the spindle fibers. Human MAD2 has also been shown to associate with insulin receptor (IR), but not IGFIR, implicating MAD2 as a mediator for IR-specific signaling. MAD2B, a MAD2 homolog, is required for the execution of the mitotic checkpoint monitoring the kinetochore-spindle attachment process and if the process is not complete, MAD2B delays the onset of anaphase.

Alternative Name:
HsMAD2, MAD2-like protein 1, MAD2L1, MAD2, Caught by MAD2 protein, CMT2, dJ261G23.1, KIAA0110, MAD2L1 binding protein, MAD2L1 binding protein isoform 2, MAD2L1-binding protein, Mad2l1bp, MD2BP, MGC11282, OTTHUMP00000016496, RP1 261G23.6,

Application Dilution:
WB:1:500-1:2,000
ICC:1:100-1:500

Specificity: Mad2L1 polyclonal antibody detects endogenous levels of Mad2L1 protein.

Immunogen:
Recombinant protein

MW: 23.5 kDa

Swiss-Prot: Q13257(Human)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.