MAG polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
MAG(Myelin-associated glycoprotein),also known as SIGLEC4A, is a cell membrane glycoprotein that is a member of the SIGLEC family of proteins and is a functional ligand of the NOGO-66 receptor, NgR. It is though to be involved in the process of myelination. MAG is a sialic acid-binding SIGLEC protein and is a functional ligand for the NOGO receptor.The MAG gene is mapped on 19q13.12. Cleavage of GPI-linked proteins from axons protects growth cones from MAG-induced collapse, and dominant-negative NgR eliminates MAG inhibition of neurite outgrowth. MAG-resistant embryonic neurons were rendered MAG-sensitive by expression of NgR. MAG binds specifically to an NgR-expressing cell line in a GPI-dependent and sialic acid-independent manner. Experiments blocking NgR from interacting with MAG prevented inhibition of neurite outgrowth by MAG. In cultured human embryonic kidney(HEK) cells expressing the NOGO receptor, p75(NTR) was required for MAG-induced intracellular calcium elevation.

Description:
Rabbit IgG polyclonal antibody for Myelin-associated glycoprotein (MAG) detection. Tested with WB, IHC-P in Human, Mouse, Rat.

Synonyms:
GMA antibody, MAG antibody, MAG_HUMAN antibody, Myelin associated glycoprotein antibody, Myelin-associated glycoprotein antibody, S MAG antibody, Siglec 4a antibody, Siglec-4a antibody, SIGLEC4A antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the N-terminus of human MAG(114-132aa KYYFRGDLGGYNQYTFSEH), identical to the related rat and mouse sequences.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, mouse, rat

Applications: WB, IHC-P

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.