MAGEA6 293 Cell Lysate
Special Price
€65.00
Regular Price
€447.00
In stock
SKU
408122
Protein Family: Cellular Antigens
Pathway & Disease: Cancers
Description:
Antigen standard for melanoma antigen family A, 6 (MAGEA6), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with aTrueORF gene-carrying pCMV plasmid (True ORF cDNA clone RC223578, OriGene Technologies, Inc.) and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Alternate Names: MAGE-6 antigen, cancer/testis antigen family 1, member 6, MAGE3B antigen, cancer/testis antigen 1.6, MAGEA6, CT1.6, MAGE-3b, MAGE3B, MAGE6, MGC52297, Melanoma-associated antigen 6
ORF clone URL:
Format: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 100 mM DTT).
Applications: ELISA, WB, IP
Accession No: NP_005354
Application Notes: WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Storages: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Pathway & Disease: Cancers
Description:
Antigen standard for melanoma antigen family A, 6 (MAGEA6), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with aTrueORF gene-carrying pCMV plasmid (True ORF cDNA clone RC223578, OriGene Technologies, Inc.) and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Alternate Names: MAGE-6 antigen, cancer/testis antigen family 1, member 6, MAGE3B antigen, cancer/testis antigen 1.6, MAGEA6, CT1.6, MAGE-3b, MAGE3B, MAGE6, MGC52297, Melanoma-associated antigen 6
ORF clone URL:
Format: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 100 mM DTT).
Applications: ELISA, WB, IP
Accession No: NP_005354
Application Notes: WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Storages: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
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