MAP Kinase, Activated (clone MAPK-YT), anti-human, mouse, rat, yeast

Background:
In mammalian cells, a variety of extracellular stimuli generate intracellular signals that converge on a limited number of so-called mitogen-activated protein(MAP) kinase pathways. The central core of each MAP kinase(MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase(MAPKKK) phosphorylates and activates a specific MAPK kinase(MAPKK), which then activates a specific MAPK. Mek1/2 MAPK kinases are essential for mammalian development, homeostasis, and Raf-induced hyperplasia. Germline mutations in genes within the MAPK pathway cause cardio-facio-cutaneous syndrome.

Decription:
Mouse IgG monoclonal antibody for MAP Kinase, Activated (Diphosphorylated ERK-1&2), mitogen-activated protein kinase 1, mitogen-activated protein kinase 3 (MAPK1, MAPK3 ) detection. Tested with WB, IHC-P, ICC in Human, mouse, rat, yeast. No cross reactivity with other proteins.

Synonyms: ERK, p38, p40, p41, ERK2, ERT1, MAPK2, PRKM1, PRKM2, P42MAPK, p41mapk, ERK1, ERT2, ERK-1, PRKM3, P44ERK1, P44MAPK, HS44KDAP, HUMKER1A, p44-ERK1, p44-MAPK, Extracellular signal-regulated kinase 2, MAP kinase isoform p42, Mitogen-activated protein kinase 2

Clone: MAPK-YT

Isotype: Mouse IgG1

Immunogen: A synthetic peptide containing 11 amino acids, HTGFLTpEYpVAT, corresponding to the phosphorylated form of ERK-activation loop conjugated to KLH.

Reactivity: human, mouse, rat, yeast

Form: Lyophilized; Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Applications: WB, IHC-P, ICC

Reconstitution: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.