MAPK8 Polyclonal Antibody

Background:
MAPK8(Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. MAPK8 is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. MAPK8 functions to phosphorylate c-Jun at N-terminal serine regulatory sites of Ser-63 and Ser-73, mapping within the transactivation domain. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun. It has 4 isoforms produced by alternative splicing with the molecular weight of 46 kDa and 48 kDa. This protein can be phosphorylated and this antibody recognizes the 46 kDa and 55 kDa bands in western blot.

Research Area: Cancer, Immunology, Signal Transduction

Synonyms:
AI849689, c Jun N terminal kinase 1, C-JUN kinase 1, c-Jun N-terminal kinase 1, EC 2.7.11.24, JNK 1, JNK, JNK-46, JNK1A2, JNK21B1/2, MAP kinase 8, MAPK 8, mapk8, Mitogen activated protein kinase 8, Mitogen-activated protein kinase 8, MK08, p54 gamma, Prkm8, Protein kinase JNK1, Protein kinase, mitogen-activated, 8, SAPK 1, SAPK gamma, SAPK1, Stress-activated protein kinase 1, Stress-activated protein kinase JNK1

Immunogen: Fusion protein of human MAPK8

Swissprot: P45983
Gene Accession: BC130570

Calculated MW: 48 kDa
Observed MW: Refer to figures

Purification Method: Antigen affinity purification

Buffer: PBS with 0.05% NaN3 and 40% Glycerol, pH7.4

Concentration: 1.9 mg/mL
Dilution: WB 1:1000-1:5000, ELISA 1:2000-1:10000

Storage:
Store at -20°C. Avoid freeze / thaw cycles.