MAST4 polyclonal, anti-human, mouse, rat

Background:
The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions in eukaryotes, including cell division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the serine/threonine(Ser/Thr) protein kinases. MAST4 (microtubule associated serine/threonine kinase family member 4), also known as KIAA0303, is a 2,626 amino acid protein that localizes to the cytoplasm and contains one protein kinase domain, as well as one PDZ domain and one AGC-kinase C-terminal domain. Expressed at high levels in most adult tissues, including testis, colon and small intestine, MAST4 uses magnesium as a cofactor to catalyze the ATP-dependent phosphorylation of target proteins. Multiple isoforms of MAST4 exist due to alternative splicing events.

Alternative Name:
Microtubule-associated serine/threonine-protein kinase 4, KIAA0303

Application Dilution: WB: 1:500~1:1000, IHC: 1:50~1:200, ICC: 1:50~1:200, IP: 1:50~1:200

Specificity: MAST4 polyclonal antibody detects endogenous levels of MAST4 protein.

Immunogen:
Synthetic peptide, corresponding to amino acids 108-153 of Human MAST4.

MW: ~ 266 kDa

Swis Prot.: O15021

Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Format:
1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.

Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

For research use only, not for use in diagnostic procedure.