MAX polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
MAX(Max protein), also called Myc-associated factor x, is the most conserved dimerization component of the MYC-MAX-MXD1 network of basic helix-loop-helix leucine zipper(bHLHZ) transcription factors that regulate cell proliferation, differentiation, and apoptosis. The conservation of the MAX sequence is particularly high in the bHLHZ domain, which is involved in protein-protein interactions and DNA binding. The MAX gene is located on chromosome 14q23 by fluorescence in situ chromosomal hybridization. Both quasisymmetric heterodimers resemble the symmetric MAX homodimer, albeit with marked structural differences in the coiled-coil leucine zipper regions that explain preferential homo- and heteromeric dimerization of these 3 evolutionarily related DNA-binding proteins. MAX acts as a classic tumor suppressor gene. Normal lymphocytes from patients showed absence of methylation of the MAX promoter and biallelic expression of MAX, which ruled out an imprinting-mediated effect on MAX expression. The ability of these cells to divide, differentiate, and apoptose in the absence of Max demonstrated for the first time that these processes can occur via Max- and possibly Myc-independent mechanisms.

Description:
Rabbit IgG polyclonal antibody for Protein max (MAX) detection. Tested with WB in Human, Mouse, Rat.

Synonyms:
bHLHd4 antibody, bHLHd5 antibody, bHLHd6 antibody, bHLHd7 antibody, bHLHd8 antibody, Helix loop helix zipper protein antibody, MAX protein antibody, MGC10775 antibody, MGC11225 antibody, MGC18164 antibody, MGC34679 antibody, MGC36767 antibody, Myc associated factor X antibody, Myc binding novel HLH/LZ protein antibody, Orf 1 antibody, Orf1 antibody, Protein max antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the N-terminus of human MAX(32-48aa ERKRRDHIKDSFHSLRD), identical to the related rat and mouse sequences.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human.
Predicted to work with: mouse, rat.

Applications: WB

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.