MBD1 polyclonal, anti-human, mouse

Quantity: 100 µg

Background:
MBD1(Methyl-CpG-Binding Domain Protein 1), also known as PCM1 or CXXC3, is a protein that in humans is encoded by the MBD1 gene. Using PCR on a hybrid panel and FISH, Hendrich et al.(1999) mapped the MBD1 gene to chromosome 18q21, 2.1 cM distal to MBD2. Using yeast 2-hybrid analysis, reciprocal immunoprecipitation analysis, and protein pull-down assays, Fujita et al.(2003) showed that MBD1 interacted directly with MCAF. Deletion analysis revealed that the C-terminal transcriptional repressor domain(TRD) of MBD1 interacted with a conserved C-terminal domain of MCAF. Reporter gene assays showed that MCAF increased the repressive function of the isolated TRD of MBD1 against SP1. Chromatin immunoprecipitation analysis revealed that MBD1 linked MCAF to methylated promoters. Uchimura et al.(2006) found that MBD1 was multiply sumoylated in HeLa cells. Sumoylation did not alter the intracellular localization of MBD1 at nuclear foci in C-33A human cervical cancer cells.

Description:
Rabbit IgG polyclonal antibody for Methyl-CpG-binding domain protein 1 (MBD1) detection. Tested with WB in Human, Mouse.

Synonyms:
CXXC 3 antibody, CXXC-type zinc finger protein 3 antibody, CXXC3 antibody, MBD 1 antibody, MBD1 antibody, MBD1_HUMAN antibody, MECP1 COMPLEX antibody, Methyl CpG binding domain protein 1 antibody, Methyl CpG binding domain protein 1 isoform PCM1 antibody, Methyl CpG binding protein MBD1 antibody, Methyl CpG binding protein splice variant 1 antibody, Methyl CpG binding protein splice variant 2 antibody, Methyl CpG binding protein splice variant 3 antibody, Methyl CpG binding protein splice variant 4 antibody, Methyl-CpG-binding domain protein 1 antibody, Methyl-CpG-binding protein MBD1 antibody, PCM 1 antibody, PCM1 antibody, Protein containing methyl CpG binding domain 1 antibody, Protein containing methyl-CpG-binding domain 1 antibody, Regulator of fibroblast growth factor 2(FGF 2) transcription antibody, RFT antibody, The regulator of fibroblast growth factor 2(FGF 2) transcription antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence in the middle region of human MBD1(366-381aa NQKRQKCRWRQCLQFA), identical to the related mouse sequence.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human.
Predicted to work with: mouse.

Applications: WB

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.