MBD1 polyclonal (C-term), anti-human
€0.00
In stock
SKU
AC-AP1036B
Catalog Number: AC-AP1036B
Size: 80 µl, 400 µl
Isotype: Rabbit IgG
Clone Name: RB2371
Applications: WB
Datasheet
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Size: 80 µl, 400 µl
Isotype: Rabbit IgG
Clone Name: RB2371
Applications: WB
Datasheet
Request Information
Background:
DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD) (1). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA (2). MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases (3). In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract (4).
Other Names:
Methyl-CpG-binding domain protein 1, CXXC-type zinc finger protein 3, Methyl-CpG-binding protein MBD1, Protein containing methyl-CpG-binding domain 1, MBD1, CXXC3, PCM1
Target/Specificity:
This MBD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 376-405 amino acids from the C-terminal region of human MBD1.
Antigen Region:
376-405
Antigen Type: Synthetic Peptide
Gene Name: MBD1 (HGNC:6916)
Gene ID: 4152
Primary Accession: Q9UIS9
NCBI Accession: NP_001191065.1;NP_001191066.1;NP_001191067.1;NP_001191068.1;NP_001191069.1;NP_001191070.1;NP_001191071.1;NP_001191072.1;NP_001191080.1;NP_002375.1;NP_056669.2;NP_056670.2;NP_056671.2;NP_056723.2
Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD) (1). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA (2). MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases (3). In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract (4).
Other Names:
Methyl-CpG-binding domain protein 1, CXXC-type zinc finger protein 3, Methyl-CpG-binding protein MBD1, Protein containing methyl-CpG-binding domain 1, MBD1, CXXC3, PCM1
Target/Specificity:
This MBD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 376-405 amino acids from the C-terminal region of human MBD1.
Antigen Region:
376-405
Antigen Type: Synthetic Peptide
Gene Name: MBD1 (HGNC:6916)
Gene ID: 4152
Primary Accession: Q9UIS9
NCBI Accession: NP_001191065.1;NP_001191066.1;NP_001191067.1;NP_001191068.1;NP_001191069.1;NP_001191070.1;NP_001191071.1;NP_001191072.1;NP_001191080.1;NP_002375.1;NP_056669.2;NP_056670.2;NP_056671.2;NP_056723.2
Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
| Is Featured? | No |
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