MBD3 polyclonal, anti-human, mouse, rat

Background:
Methylation of DNA contributes to the regulation of gene transcription in both mammalian and invertebrate systems. DNA methylation predominates on cytosine residues that are present in dinucleotide motifs consisting of a 5′ cytosine followed by guanosine (CpG), and it requires the enzymatic activity of DNA methyltransferase, which results in transcriptional repression of the methylated gene. Several proteins have been identified that associate with the methyl-CpG sites, and they include methyl-CpG binding protein-1 (MBD1), MBD2, MBD3, MBD4 and MeCP2. Expression of the MBD proteins is highest in somatic tissues.

Alternative Name:
MBD3, AI181826, AU019209, MBD 3, Mbd3, MBD3: methyl CpG binding domain protein 3, MBD3, Methyl CpG binding domain protein 3, Methyl CpG binding protein MBD3, Methyl-CpG-binding domain protein 3, Methyl-CpG-binding protein MBD3,

Application Dilution:
WB:1:500
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Specificity: MBD3 polyclonal antibody detects endogenous levels of MBD3 protein.

Immunogen:
Recombinant protein

MW: 33 kDa

Swiss-Prot: O95983(Human) Q9Z2D8(Mouse)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.