MBD4 polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
MBD4(Methyl-CpG-Binding Domain Protein 4), also known as MED1, is a protein that in humans is encoded by the MBD4 gene. MBD4 specifically binds methylated DNA, colocalizes with methylated sequences, and is likely to mediate the effects of DNA methylation in mammalian cells(Hendrich and Bird, 1998). Riccio et al.(1999) mapped the MBD4 gene to chromosome 3q21-q22 by FISH. Hendrich and Bird(1998) found that both MBD2 and MBD4 specifically bound methylated DNA in vitro and colocalized with methylated sequences in vivo. They concluded that MBD2 and MBD4 are likely to be mediators of the effects of DNA methylation in mammalian cells. Hendrich et al.(1999) showed that MBD4 contains a methyl-CpG-binding domain that can efficiently remove thymine or uracil from mismatched CpG sites in vitro. Furthermore, the methyl-CpG-binding domain of MBD4 binds preferentially to 5-methylcytosine CpG-TpG mismatches--the primary product of deamination at methyl-CpG.

Description:
Rabbit IgG polyclonal antibody for Methyl-CpG-binding domain protein 4 (MBD4) detection. Tested with WB in Human, Mouse, Rat.

Synonyms:
3 N(4) ethenocytosine glycosylase antibody, G/5 fluorouracil mismatch glycosylase with biphasic kinetics antibody, G/T mismatch glycosylase antibody, G/U mismatch glycosylase antibody, MBD 4 antibody, MBD4 antibody, MBD4_HUMAN antibody, MED 1 antibody, MED1 antibody, Methyl CpG binding domain protein 4 antibody, Methyl CpG binding endonuclease 1 antibody, Methyl CpG binding protein MBD4 antibody, Methyl-CpG-binding domain protein 4 antibody, Methyl-CpG-binding endonuclease 1 antibody, Methyl-CpG-binding protein MBD4 antibody, Mismatch specific DNA N glycosylase antibody, Mismatch-specific DNA N-glycosylase antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the C-terminus of human MBD4(566-580aa YHDWLWENHEKLSLS), identical to the related rat and mouse sequences.A synthetic peptide corresponding to a sequence at the C-terminus of human MBD4(566-580aa YHDWLWENHEKLSLS), identical to the related rat and mouse sequences.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, rat .
Predicted to work with: mouse.

Applications: WB

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.