MCM4 polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
MCM4(MINICHROMOSOME MAINTENANCE, S. CEREVISIAE, HOMOLOG OF, 4), also called CDC21, S. POMBE, HOMOLOG OF, is a protein that in humans is encoded by the MCM4 gene. MCM4 is one of the highly conserved mini-chromosome maintenance proteins(MCM) that are essential for the initiation of eukaryotic genome replication. The MCM4 gene is mapped to 8q11.21. The 864-amino acid MCM4 protein has an observed molecular mass of 97 kD by SDS-PAGE, which is similar to its calculated molecular mass of 96.6 kD. Western blot analysis with and without phosphatase treatment suggested that MCM4 is highly phosphorylated in mitotic cells. In the absence of DDK, CDK phosphorylation at the distal part of the Mcm4 NSD becomes crucial.MCM4 encodes a subunit of the MCM2-7 complex(also known as MCM2-MCM7), the replication licensing factor and presumptive replicative helicase.

Description:
Rabbit IgG polyclonal antibody for DNA replication licensing factor MCM4 (MCM4) detection. Tested with WB in Human, Mouse, Rat.

Synonyms:
19G antibody, AI325074 antibody, AU045576 antibody, CDC 21 antibody, CDC 54 antibody, CDC21 antibody, CDC21 homolog antibody, CDC21, S. pombe, homolog of antibody, CDC54 antibody, Cell division cycle 21, S. pombe, homolog of antibody, DNA replication licensing factor MCM 4 antibody, DNA replication licensing factor MCM4 antibody, hCdc 21 antibody, hCdc21 antibody, Homolog of S. pombe cell devision cycle 21 antibody, KIAA4003 antibody, mcdc21 antibody, MCM 4 antibody, MCM 4 minichromosome maintenance deficient 4 antibody, MCM4 antibody, MCM4 minichromosome maintenance deficient 4 antibody, MCM4 minichromosome maintenance deficient 4, mitotin(S. cerevisiae) antibody, MCM4_HUMAN antibody, Mcmd4 antibody, MGC33310 antibody, Minichromosome Maintenance 4 antibody, Minichromosome maintenance complex component 4 antibody, Minichromosome maintenance deficient(S. cerevisiae) 4 antibody, Minichromosome maintenance deficient 4 antibody, Minichromosome maintenance deficient 4 homolog(S. cerevisiae) antibody, Minichromosome maintenance, S. cerevisiae, homolog of, 4 antibody, mKIAA4003 antibody, P1 CDC21 antibody, P1-CDC21 antibody, P1CDC21 antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the C-terminus of human MCM4(815-829aa TPALKYQQLFEDIRG), identical to the related rat and mouse sequences.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human.
Predicted to work with: mouse, rat.

Applications: WB

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.