MCP-1 mouse active recombinant protein

MCP-1 mouse active recombinant protein

€295.00
In stock
SKU
600363
Catalog Number: 600363
Size: 0.01 mg
Datasheet
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Protein Family: Cytokines

Pathway and Disease: Cancers, Immune System, Signal Transduction, Signaling Molecules and Interaction

Alternate Names: C-C motif chemokine 2, small-inducible cytokine A2, MCP-1, monocyte chemoattractant protein-1, monocyte chemotactic protein 1, monocyte chemotactic and activating factor, MCAF, monocyte secretory protein JE, HC11, CCL2, MCP1, SCYA2Description:
Monocyte Chemotactic Protein 1 (MCP-1), also known as CCL2, is thought to be produced by injured or infected tissues. MCP-1 signals through G protein-coupled receptors, CCR2 and CCR4, to recruit memory T cells, monocytes and dendritic cells. Mouse MCP-1 is produced in E.coli. Recombinant mouse MCP-1 is a non-glycosylated protein, containing 125 amino acids, with a molecular weight of 13.8 kDa.

Source: E. coli. Endotoxin level as measured by LAL is <0.05ng/ug or <0.5EU/ug.

Applications: E, WB

Application Notes:
The activity is determined by its ability to chemoattract human PBMCs or THP-1 cells at 4 - 15 ng/mL. Specific activity: 2.5 x 10e5 units/mg

Accession No.: P10148

MW: 13800 Da

Sequence:
QPDAVNAPLT CCYSFTSKMI PMSRLESYKR ITSSRCPKEA VVFVTKLKRE VCADPKKEWV QTYIKNLDRN QMRSEPTTLF KTASALRSSA PLNVKLTRKS EANASTTFST TTSSTSVGVT SVTVN

Purity:
Purity > 95% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.

Format:
Each vial contains 10 ug of lyophilized protein. Reconstitute with 0.1 ml sterile deionized water for a final concentration of 0.1 mg/ml.

Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
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