MCP-4 monoclonal, anti-human
€0.00
In stock
SKU
AC-ABG10373
Catalog Number: AC-ABG10373
Size(s): 500 µg
Host: Mouse
Applications: WB, E
Datasheet
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Size(s): 500 µg
Host: Mouse
Applications: WB, E
Datasheet
Request Information
Source:
Produced in BALB/c mice using highly pure (>98%) recombinant human MCP-4 as the immunizing antigen. This IgG1k antibody was purified from cell culture by Protein A affinity chromatography.
Western Blot:
To detect hMCP-4 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-4 is 2.0-4.0 ng/lane, under non-reducing conditions.
Sandwich ELISA:
In a sandwich ELISA (assuming 100 μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 100 pg/ml of recombinant human MCP-4 when used with BioGems biotinylated antigen affinity purified anti-human MCP-4 (60-215BT) as the detection antibody at a concentration of approximately 0.5-1.0 μg/ml.
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Human MCP-4 (100 ng/ml), a concentration of 0.8-1.0 μg/ml of this antibody is required.
Formulation: A sterile filtered antibody solution was lyophilized from PBS.
Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Produced in BALB/c mice using highly pure (>98%) recombinant human MCP-4 as the immunizing antigen. This IgG1k antibody was purified from cell culture by Protein A affinity chromatography.
Western Blot:
To detect hMCP-4 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-4 is 2.0-4.0 ng/lane, under non-reducing conditions.
Sandwich ELISA:
In a sandwich ELISA (assuming 100 μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 100 pg/ml of recombinant human MCP-4 when used with BioGems biotinylated antigen affinity purified anti-human MCP-4 (60-215BT) as the detection antibody at a concentration of approximately 0.5-1.0 μg/ml.
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Human MCP-4 (100 ng/ml), a concentration of 0.8-1.0 μg/ml of this antibody is required.
Formulation: A sterile filtered antibody solution was lyophilized from PBS.
Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
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