MDA5 (clone Hely-1), anti-human

Decription:
RIG-I and MDA5 are highly conserved helicases involved in the innate immune response to virus. RIG-I is a member of the DEAD-box RNA helicases and is activated by cytoplasmic dsRNA and 5'-ppp RNA produced during the viral replication. The protein is characterized by a N-terminal region with two caspase recruitment domains (CARD) and a C-terminal region harboring potential ATP-dependent RNA helicase activity. RIG-1 recruits the CARD adaptor inducing IFN-beta (Cardif) in a CARD-CARD-dependent manner resulting in NF-kappaB and IRF3 activation.

Synonyms: Helicard, Melanoma Differentiation-associated Gene 5

Clone: Hely-1

Isotype: Mouse IgG1

Immunogen: Recombinant human MDA5 (aa 78-555).

Reactivity: human

Specificity: Recognizes human MDA5.

Form: Purified, in PBS containing 10% glycerol and 0.02% sodium azide.

Applications:
ELISA
Immunoprecipitation: (1:100) Western Blot: (1:1‘000)

Working Dilution: Optimal conditions must be determined individually for each application.

Concentration: 1mg/ml

Storage: 2-8C immediate use, or -20C (aliquote)