MDM2 polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
Mdm2 is an important negative regulator of the p53 tumor suppressor. It is the name of a gene as well as the protein encoded by that gene. Mdm2 protein functions both as an E3 ubiquity lipase that recognizes the N-terminal trans-activation domain(TAD) of the p53 tumor suppressor and an inhibitor of p53 transcriptional activation. Oliner et al.(1992) used MDM2 clones to localize the human gene to chromosome 12q13-q14 by analysis of human-hamster somatic cell hybrids.

Description:
Rabbit IgG polyclonal antibody for E3 ubiquitin-protein ligase Mdm2 (MDM2) detection. Tested with WB in Human, Mouse, Rat.

Synonyms:
Double minute 2 protein antibody, E3 ubiquitin-protein ligase Mdm2 antibody, Hdm 2 antibody, HDM2 antibody, MDM 2 antibody, MDM2 antibody, Mdm2 transformed 3T3 cell double minute 2 p53 binding protein(mouse) binding protein 104kDa antibody, MDM2_HUMAN antibody, MDM2BP antibody, Mouse Double Minute 2 antibody, MTBP antibody, Murine Double Minute Chromosome 2 antibody, Oncoprotein Mdm2 antibody, p53 Binding Protein Mdm2 antibody, p53-binding protein Mdm2 antibody, Ubiquitin protein ligase E3 Mdm2 antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the C-terminus of human MDM2(343-358aa EKAKLENSTQAEEGFD), different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, mouse, rat

Applications: WB

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.