MEK-7 (phospho-S271) polyclonal antibody, anti-human, mouse, rat

Background:
MKK7 is a MAP kinase kinase that serves as a specific activator of the SAPK/JNK pathway. MKK7 is strongly activated by TNF-α, as well as other environmental stresses, whereas SEK1/MKK4, which activates both p38 and SAPK/JNK pathways, is not activated by TNF-α. Sequence alignment of the activation loop of the MAP kinase kinase family members indicates that Ser271 and Thr275 are potential phosphorylation sites that are crucial for the kinase acivity.

Alternative Name:
Mitogen Activated Protein Kinase kinase 7, c-Jun N-terminal kinase kinase 2, JNK activating kinase 2, Jnkk 2, Jnkk2, MAP2K7, MAPKK-7, MKK 7, MKK-7, PRKMK 7, PRKMK7, Sek-2, Dual specificity mitogen activated protein kinase kinase 7, JNK kinase 2, Jnkk

Application Dilution:
WB: 1:500~1:1000

Specificity: MEK-7 (phospho-S271) polyclonal antibody detects endogenous levels of MEK-7 protein only when phosphorylated at Ser271.

Immunogen:
Synthetic phosphopeptide derived from human MEK-7 around the phosphorylation site of Serine 271

MW: ~ 48 kDa

Swiss-Prot: O14733

Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Format:
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.3.

Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

For research use only, not for use in diagnostic procedure.