MEKK1 polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
MAP3K1(Mitogen-activated protein kinase kinase kinase 1), also known as MEKK1, MAPKKK1, MEK KINASE or MAP/ERK KINASE KINASE 1, is an enzyme that in humans is encoded by the MAP3K1 gene. Vinik et al.(1995) identified DNA sequence and size polymorphisms in intronic and 3-prime untranslated regions of the mouse Map3k1 gene and the human MAP3K1 homolog. Using these allele-specific polymorphisms, they mapped the Map3k1 gene in an intersubspecific backcross to mouse chromosome 13. They mapped the human MAP3K1 gene to chromosome 5 by somatic cell hybrid analysis. By assaying transfected COS-1 cells, Xia et al.(1998) showed that human MEKK1 activated JNK1(MAPK8) robustly and p38-alpha(MAPK14) less efficiently, but it had only a marginal effect on ERK2(MAPK1). MEKK1 directly and specifically interacted with JNKK1(MAP2K4) and activated JNKK1 in cells and in vitro. Phosphorylation of JNKK1 by MEKK1 disrupted their interaction. MEKK1 and JNK1 competed for binding to JNKK1. Xia et al.(1998) concluded that JNKK1 is the preferred MEKK1 substrate.

Description:
Rabbit IgG polyclonal antibody for Mitogen-activated protein kinase kinase kinase 1 (MAP3K1) detection. Tested with WB, IHC-P in Human, Mouse, Rat.

Synonyms:
M3K1_HUMAN antibody, MAP3K1 antibody, MAPK/ERK kinase kinase 1 antibody, MAPKKK1 antibody, MEK kinase 1 antibody, MEKK 1 antibodyMekk antibody, MEKK1 antibody, Mitogen activated protein kinase kinase kinase 1 antibody, Mitogen activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase antibody, Mitogen-activated protein kinase kinase kinase 1 antibodySRXY6 antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the C-terminus of human MEKK1(1418-1432aa PEVLRGQQYGRSCDV), identical to the related rat and mouse sequences.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human.
Predicted to work with: mouse, rat.

Applications: WB, IHC-P

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.